A plasmid that has genes for both tetracycline resistance and ampicillin resistance is cut with the restriction enzyme BamHI.BamHI cuts within the ampicillin resistance gene.Caenorhabditis elegans DNA that has been cut with BamHI is ligated into the linearized plasmid to make a genomic DNA library.The mixture is transformed into
A)Both ampicillin and tetracycline are needed to make this determination.
B)tetracycline
C)Neither ampicillin or tetracycline are needed to make this determination.
D)ampicillin
E)coli.What antibiotic(s),if any,should be added to the medium to determine which cells contain plasmids?

Question

Quizplus · Accepted Answer

Since BamHI cuts within the ampicillin resistance gene, disrupting its function, only the tetracycline resistance gene remains functional in the plasmid. Therefore, adding tetracycline to the medium will allow selection of cells that contain the plasmids, as these cells will be resistant to tetracycline.

A Plasmid That Has Genes for Both Tetracycline Resistance and Ampicillin