You transform bacteria with a plasmid carrying the ampicillin-resistance gene amp^R.How would you determine which bacteria took up the plasmid? A)Bacteria containing the plasmid would be able to grow in the absence of ampicillin. B)Ampicillin-resistance is necessary for the plasmid DNA to get into the bacteria. C)Bacteria containing the plasmid would be killed in the presence of ampicillin. D)Bacteria containing the plasmid would be able to produce ampicillin. E)Bacteria containing the plasmid would be able to grow in the presence of ampicillin.

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Quizplus · Accepted Answer

The plasmid carries the ampicillin-resistance gene, which will allow bacteria that have taken up the plasmid to grow in the presence of ampicillin. Therefore, the best way to determine which bacteria have taken up the plasmid is to grow them on media containing ampicillin. Only the bacteria that contain the plasmid will be able to grow in the presence of ampicillin.

You Transform Bacteria with a Plasmid Carrying the Ampicillin-Resistance Gene