What is the most likely consequence of using a plasmid that lacks an origin of replication for a gene cloning project?
A)No recombinant plasmids will be created because the plasmid is only capable of re-circularizing.
B)Bacteria containing the plasmid will not be able to grow in the presence of antibiotics.
C)The plasmid will not be cut with restriction enzymes.
D)Multiple copies of the gene of interest will not be produced because the plasmid will be lost during bacterial division.
E)The plasmid will not be useful in producing a protein because it will lack an RNA polymerase binding sitE.

Question

Quizplus · Accepted Answer

Without an origin of replication, the plasmid will not be able to replicate itself, and therefore will not be passed on to daughter cells during division. This means that multiple copies of the gene of interest will not be produced, and the plasmid may be lost entirely in subsequent generations of bacteria.

What Is the Most Likely Consequence of Using a Plasmid