A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme.The digested plasmid and chromosomal DNA were incubated together with DNA ligase and transformed into bacteria.Plasmids isolated from some of the colonies do not contain inserted chromosomal DNA.What is the most likely explanation for this observation?
A)The chromosomal DNA did not have any restriction sites for the enzyme used.
B)The two sticky ends of the plasmid can hybridize to both sticky ends of the chromosomal DNA fragments.
C)The two sticky ends of the plasmid can hybridize back together,recircularizing.
D)The two sticky ends of the vector can remain free or unhybridizeD.
E)The two sticky ends of the plasmid can hybridize with only one end of the chromosomal DNA fragments

Question

Quizplus · Accepted Answer

The plasmid vector could have recircularized without inserting any chromosomal DNA, especially if the DNA was not treated with an additional enzyme to prevent self-ligation of the plasmid.

A Plasmid Vector and Chromosomal DNA Are Treated Separately with the Same