You analyze some DNA using an automated sequence reaction.The first peak you observe is labeled with a red fluorescent label.From this you can conclude that the most ____ base in the DNA is a _____ containing the red fluorescent label.
A)5',dideoxynucleotide
B)3',deoxynucleotide
C)5',deoxynucleotide
D)3',dideoxynucleotide

Question

Quizplus · Accepted Answer

In automated DNA sequencing, dideoxynucleotides are used to terminate DNA synthesis. The color of the fluorescent label indicates which base is incorporated. Since DNA synthesis occurs in the 5' to 3' direction, the first base observed (with the red label) would be at the 3' end of the synthesized strand, making it a 3', dideoxynucleotide.

You Analyze Some DNA Using an Automated Sequence Reaction