You are working in a cancer lab and are interested in identifying some genes that are important in the onset of lung cancer.You have several genes you think may be involved,so you "print" spots of the DNA sequences of interest on a microarray slide.After doing this,you isolate mRNA from both normal lung cells and cancerous lung cells.Before you use these mRNAs to probe the microarray slide,what do you need to do?
A)make cDNA and amplify the cDNA using PCR
B)sequence the mRNA
C)clone each mRNA into a BAC vector
D)amplify the mRNA using PCR
E)add reverse transcriptase and fluorescent nucleotides to the mRNA to make cDNA

Question

Quizplus · Accepted Answer

To probe the microarray slide, mRNA must first be converted to cDNA using reverse transcriptase and fluorescent nucleotides. This will allow for the detection and measurement of gene expression levels in the normal and cancerous lung cells. There is no need to amplify the mRNA using PCR since this can introduce bias, and sequencing or cloning each mRNA into a BAC vector would be too time-consuming and costly.

You Are Working in a Cancer Lab and Are Interested