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Shown Below Is a Diagram for a Plasmid Vector You

Question 9

Multiple Choice

Shown below is a diagram for a plasmid vector you want to use to clone a gene.The diagram shows the location of the recognition sites for four restrictions enzymes,BamHI (B) ,EcoRI (E) ,HindIII (H) ,and XhoI (X) .The genes encoding beta-lactamase (AmpR) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning? Shown below is a diagram for a plasmid vector you want to use to clone a gene.The diagram shows the location of the recognition sites for four restrictions enzymes,BamHI (B) ,EcoRI (E) ,HindIII (H) ,and XhoI (X) .The genes encoding beta-lactamase (Amp<sup>R</sup>) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning?   A) BamHI B) EcoRI C) HindIII D) XhoI E) BamHI or XhoI would be equally useful.


A) BamHI
B) EcoRI
C) HindIII
D) XhoI
E) BamHI or XhoI would be equally useful.

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