Shown below is a diagram for a plasmid vector you want to use to clone a gene.The diagram shows the location of the recognition sites for four restrictions enzymes,BamHI (B) ,EcoRI (E) ,HindIII (H) ,and XhoI (X) .The genes encoding beta-lactamase (AmpR) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning?
A) BamHI
B) EcoRI
C) HindIII
D) XhoI
E) BamHI or XhoI would be equally useful.
Correct Answer:
Verified
Q4: After a fragment of DNA containing the
Q5: You cut some sheep DNA and some
Q6: What is TRUE of restriction enzymes?
A)they randomly
Q7: The lacZ gene encodes
A)aromatase
B)glucose dehydrogenase
C)ampicillin-resistance protein
D)b-galactosidase
E)lactose
Q8: What is true of cDNA produced using
Q10: You transform bacteria with a plasmid carrying
Q11: Which of the following stretches of double-stranded
Q12: Gene cloning
A)is the process of making multiple
Q13: Restriction enzymes are invaluable tools in gene
Q14: A plasmid that has genes for both
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