After a fragment of DNA containing the gene of interest has been inserted into a vector,how are the gaps between the two pieces of DNA sealed together?
A)DNA polymerase catalyzes the formation of hydrogen bonds in the DNA backbone.
B)DNA gyrase creates tension in the DNA backbone to hold the molecule together.
C)DNA helicase causes the double helix to wind into its stable form.
D)DNA ligase catalyzes the formation of covalent bonds in the DNA backbone.
E)Restriction enzymes have the ability to join the two DNA fragments.

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme that catalyzes the formation of covalent bonds between the sugar and phosphate backbone of the two DNA fragments, sealing the gaps between them. This process is called ligation. DNA polymerase, DNA gyrase, DNA helicase, and restriction enzymes do not have the ability to seal gaps between two DNA fragments in a vector.

After a Fragment of DNA Containing the Gene of Interest