What is the purpose of RT(reverse transcriptase)-PCR?
A) to conduct a PCR reaction without the use of primers
B) to follow the amount of a specific PCR product in real time
C) to quantify RNA in a cell
D) to nonspecifically amplify DNA

Question

Quizplus · Accepted Answer

The purpose of RT-PCR is to convert RNA into cDNA (complementary DNA) through reverse transcription and then to quantitate the amount of RNA present in a sample. This method is used to measure gene expression levels because it allows for the detection of RNA in small amounts. Choice A is incorrect because primers are still required in RT-PCR. Choice B is referring to real-time PCR or quantitative PCR, which is a different method altogether. Choice D is incorrect because RT-PCR is not used to amplify DNA nonspecifically.

What Is the Purpose of RT(reverse Transcriptase)-PCR