DNA ligase is needed in a cloning experiment for what reason?
A) to proofread the vector for mistakes
B) to reestablish the stable sugar-phosphate backbone of the DNA molecule
C) to allow the vector to enter into the cell
D) as a selectable marker

Question

Quizplus · Accepted Answer

DNA ligase is used in a cloning experiment to reestablish the stable sugar-phosphate backbone of the DNA molecule by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. This is necessary to join the vector DNA with the insert DNA during the cloning process.

DNA Ligase Is Needed in a Cloning Experiment for What