Select the technique that can be used to see how a specific mutation affects the expression of a gene and the function of a protein.
A) site-directed mutagenesis
B) Western blotting
C) real-time PCR
D) DNA sequencing

Question

Quizplus · Accepted Answer

Site-directed mutagenesis allows for the precise alteration of a specific nucleotide sequence in DNA. This technique can be used to introduce specific mutations in a gene of interest, and then observe how these mutations affect gene expression and protein function. This is useful for studying the impact of individual mutations on gene and protein function. Western blotting, real-time PCR, and DNA sequencing, while all valuable techniques, are not as directly applicable to seeing the effects of a specific mutation on gene expression and protein function.

Select the Technique That Can Be Used to See How