What is the fluorescence resonance energy transfer (FRET) methodology?
A) The transfer of energy is made from a donor dye molecule to an acceptor dye molecule.
B) A fluorescent probe is mixed with labeled primers and a sandwich occurs where the fluorescent molecule is activated to fluorescent.
C) A light shines on the fluorescent probe label and it fluoresces.
D) A chromogenic substrate is mixed into solution, and when it combines with the Taq DNA polymerase, a fluorophore is formed and it fluoresces.

Question

Quizplus · Accepted Answer

Fluorescence resonance energy transfer (FRET) is a method of measuring the distance between two fluorescent molecules. The transfer of energy is made from a donor molecule (usually a fluorescent dye) to an acceptor molecule (also usually a fluorescent dye) when they are in close proximity. This phenomenon allows researchers to study molecular interactions and structural changes in biological systems.

What Is the Fluorescence Resonance Energy Transfer (FRET) Methodology