What Is the Principle of Restriction Enzyme Analysis of Chromosomal

What is the principle of restriction enzyme analysis of chromosomal DNA?

A) Plasmids are cut into small pieces with restriction enzymes. The resulting restriction fragment length polymorphism (RFLP) pattern is analyzed by agarose gel electrophoresis; there is transfer to a membrane, then use of a probe to identify specific sequences.
B) The chromosomal DNA is denatured, then annealed. The resulting hybrids are electrophoresed, transferred to a membrane, and then analyzed for a specific sequence.
C) DNA is extracted and isolated, and digested with a restriction enzyme. The resulting RFLP pattern is analyzed by agarose gel electrophoresis and transferred to a membrane.
D) The serum specimen is electrophoresed, transferred to a membrane, digested, and then reacted with a probe to identify the target.