The most sensitive technique available for detecting and quantifying messenger RNA is
A) multiplex PCR
B) standard PCR
C) nested PCR
D) reverse-transcription PCR

Question

Quizplus · Accepted Answer

Reverse-transcription PCR (RT-PCR) is the most sensitive technique available for detecting and quantifying messenger RNA (mRNA). RT-PCR involves the conversion of mRNA to complementary DNA (cDNA) through the use of reverse transcriptase enzyme, followed by amplification of cDNA using PCR. This allows for the detection and quantification of specific mRNA transcripts in a sample. Multiplex PCR, standard PCR, and nested PCR do not involve the conversion of mRNA to cDNA and are not as sensitive for mRNA detection and quantification.

The Most Sensitive Technique Available for Detecting and Quantifying Messenger