During the denaturation step in PCR
A) DNA polymerase extends the primers.
B) oligonucleotide primers are hybridized to the single-stranded DNA.
C) native DNA is cut into small pieces with a restriction enzyme.
D) the target double-stranded DNA is separated into single strands.

Question

Quizplus · Accepted Answer

During the denaturation step in PCR, the target double-stranded DNA is separated into single strands through the use of heat or a denaturing agent, such as sodium hydroxide or urea. This allows the primers to anneal (hybridize) to each of the single strands.

During the Denaturation Step in PCR