Which of the following is FALSE regarding P element- mediated transformation?
A) The gene of interest is typically inserted in the vector plasmid between the P element inverted repeat end sequences.
B) The selectable marker is typically inserted in the vector plasmid between the P element inverted repeat end sequences.
C) The transposase activity inserts P elements into the genome at specific positions in the genome.
D) P elements transpose only in the germ- line cells of Drosophila.
E) A second plasmid can be used to supply the transposase activity in trans.
Correct Answer:
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