Which of the following is true regarding the Cre- lox recombination system?
A) Cre recombinase can recognize the P element inverted repeat end sequences.
B) This site- specific recombination system is derived from Drosophila.
C) Cre recombinase initiates conjugative transfer of the T- DNA in Agrobacterium tumefaciens.
D) It can be used to remove selectable markers in transgenic organisms.
E) It is limited to manipulating DNA sequences in vitro.

Question

Quizplus · Accepted Answer

The Cre-lox recombination system, derived from bacteriophage P1, allows for site-specific recombination events to occur within an organism's DNA. Cre recombinase recognizes loxP sites, specific DNA sequences, to catalyze the excision, insertion, or inversion of DNA segments between these sites. This system is widely used in genetic engineering, including the removal of selectable marker genes from transgenic organisms, making option D correct. Options A, B, and C are incorrect as they describe characteristics of other systems or organisms. Option E is also incorrect because the Cre-lox system is extensively used for manipulating DNA both in vitro and in vivo, not limited to in vitro applications.

Which of the Following Is True Regarding the Cre- Lox