Half of the palindromic restriction site that the endonuclease PstI recognizes, binds, and cleaves is 5'- CTG _______- 3'. What is the complete sequence?
A) 5'- CTGCAG- 3'
B) 5'- CTGGTC- 3'
C) 5'- CTGCTG- 3'
D) 5'- CTGGAG- 3'
E) 5'- CTGGAC- 3'

Question

Quizplus · Accepted Answer

PstI recognizes and cleaves the palindromic sequence 5'-CTGCAG-3'. The complementary sequence to 5'-CTG-3' on the opposite strand must be 3'-GAC-5', which when read in the 5' to 3' direction is 5'-CAG-3', making the complete sequence 5'-CTGCAG-3'.

Half of the Palindromic Restriction Site That the Endonuclease PstI