Which of the following make(s) sequencing by the Sanger chain-termination method possible?
A)New nucleotides are added only to the 3' end of a growing DNA strand.
B)A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated.
C)Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule.
D)Duplex nucleic acid molecules can be separated by size by means of electrophoresis.
E)Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper.

Question

Quizplus · Accepted Answer

The Sanger method relies on the addition of nucleotides to the 3' end (A), termination of elongation by dideoxynucleotides (B), formation of duplexes for sequencing (C), and size separation by electrophoresis (D).

Which of the Following Make(s) Sequencing by the Sanger Chain-Termination