Which of the following make(s) amplification of a DNA fragment by means of the polymerase chain reaction possible?
A)Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule.
B)Duplex nucleic acid molecules can be separated by size by means of electrophoresis.
C)A restriction enzyme cleaves duplex DNA molecules only at the positions of certain short sequences constituting the enzyme's restrictions site.
D)DNA polymerase elongates a growing DNA strand by the addition of successive deoxynuclotides to the 3' end.
E)DNA ligase is an enzyme that can join the ends of single-stranded DNA molecules.

Question

Quizplus · Accepted Answer

Complementary sequences allow primers to bind to the target DNA, and DNA polymerase extends the DNA strand by adding nucleotides to the 3' end, enabling PCR amplification.

Which of the Following Make(s) Amplification of a DNA Fragment