The Southern blotting technique requires:
A)complementarity between the nucleotide sequence of the probe DNA and the DNA to be detected.
B)DNA primers.
C)complementarity between the protein being expressed and the radioactive probe.
D)separation of proteins by size.
E)None of the other answer options is correct.

Question

Quizplus · Accepted Answer

The Southern blotting technique requires complementarity between the nucleotide sequence of the probe DNA and the DNA to be detected. This specificity allows for the probe to bind to the DNA of interest and for detection of the target sequence. DNA primers are used in PCR and not in Southern blotting. Complementarity between the protein being expressed and the radioactive probe is not a requirement for Southern blotting, as it is used for detecting nucleotide sequences only. Separation of proteins by size is not necessary for this technique.

The Southern Blotting Technique Requires