To calculate the turnover number of an enzyme, you need to know: A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] K_m. C) the initial velocity of the catalyzed reaction at low [S]. D) the K_m for the substrate. E) both the enzyme concentration and the initial velocity of the catalyzed reaction at [S] K_m.

Question

To calculate the turnover number of an enzyme, you need to know: A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] >> K_m. C) the initial velocity of the catalyzed reaction at low [S]. D) the K_m for the substrate. E) both the enzyme concentration and the initial velocity of the catalyzed reaction at [S] >> K_m.

Quizplus · Accepted Answer

The turnover number of an enzyme is calculated by dividing the initial velocity of the catalyzed reaction at saturating substrate concentration ([S] >> Km) by the enzyme concentration. This requires knowledge of both the enzyme concentration and the initial velocity under these conditions.

To Calculate the Turnover Number of an Enzyme, You Need