Which of the following solutions would be most likely to completely unfold a protein?
A) a dilute phosphate buffer at 100 degrees Celsius
B) 5M guanidine hydrochloride
C) one percent sodium dodecyl sulfate
D) five percent trichloroacetic acid
E) twenty percent acetone

Question

Quizplus · Accepted Answer

Guanidine hydrochloride is a strong denaturant that disrupts protein structure by disrupting hydrogen bonding and hydrophobic interactions. This often results in the complete unfolding of the protein. The other options listed may denature the protein to some extent, but they are less likely to completely unfold it.

Which of the Following Solutions Would Be Most Likely to Completely