Protein separation techniques which utilize differences in charge between proteins in a mixture are:
A) Carboxymethyl (CM) chromatography
B) Affinity chromatography
C) Isoelectric focusing
D) A and B
E) A and C

Question

Quizplus · Accepted Answer

Carboxymethyl (CM) chromatography utilizes differences in charge between proteins in a mixture by using a column with a negatively charged resin. Affinity chromatography separates proteins based on their specific interactions with a ligand. Isoelectric focusing separates proteins based on their isoelectric point, which is determined by their charge. Therefore, both A and C are protein separation techniques that utilize differences in charge between proteins in a mixture. B, on the other hand, uses specific interactions between ligands and proteins for separation. Therefore, the best choice in this case is E - A and C.

Protein Separation Techniques Which Utilize Differences in Charge Between Proteins