Primers are used in both PCR and in DNA sequencing.What is the purpose of the primers?
A)to provide a 3'- OH for DNA polymerase to build a new DNA strand
B)to terminate synthesis of the growing DNA strand
C)to add nucleotides to a growing DNA strand
D)to separate the DNA template strands
E)to separate various-sized DNA products

Question

Quizplus · Accepted Answer

The purpose of primers is to provide a 3'- OH for DNA polymerase to begin building a new DNA strand. This is necessary because DNA polymerase can only add nucleotides to an existing 3'- OH group. Therefore, primers anneal to the template DNA strand and provide the necessary starting point for polymerization.

Primers Are Used in Both PCR and in DNA Sequencing