You have the target DNA and primers shown below.You add the target DNA and primers,along with dATP,dTTP,dGTP,dCTP and Taq DNA polymerase to a test tube.After 30 cycles of PCR,which of the following is true about the DNA in your test tube?
Target DNA: 5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
Primer 1: 5' - GATCAA - 3'
Primer 2: 5' - CGGAAA - 3'
A)There will be around a billion DNA molecules and most will look just like the template
5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
B)There will be around a billion DNA molecules and most will be single stranded,either
5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3' OR
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
C)Only the template and primers will be present;since no DNA helicase was added the strands won't separate so no new DNA can be made
D)There will be around a billion DNA molecules and most will contain the region that is flanked by the primers
5' - GATCAATCAATGCCGAATTTCCG - 3'
3' - CTAGTTAGTTACGGCTTAAAGGC - 5'
E)There will be around a million DNA molecules of various sizes,depending on where chain termination occurred

Question

Quizplus · Accepted Answer

After 30 cycles of PCR, there will be around a billion DNA molecules and most will contain the region that is flanked by the primers, which is 5' - GATCAATCAATGCCGAATTTCCG - 3' and 3' - CTAGTTAGTTACGGCTTAAAGGC - 5'. The primers will anneal to the target DNA and Taq DNA polymerase will replicate the region between the primers, resulting in most of the amplified DNA molecules containing this region.

You Have the Target DNA and Primers Shown Below