Why is Taq polymerase used in a polymerase chain reaction (PCR)?
A)Taq polymerase has proofreading activity and thus makes fewer errors than other DNA polymerases.
B)Taq polymerase is more efficient than other DNA polymerases.
C)Taq polymerase can produce DNA from an RNA template,unlike other DNA polymerases.
D)Unlike other DNA polymerases,Taq polymerase is not inhibited by dideoxy nucleotides.
E)Unlike other DNA polymerases,Taq polymerase is heat stable and survives the 94 degree denaturation step in PCR.

Question

Quizplus · Accepted Answer

Taq polymerase is heat stable and can survive the high temperatures used in the denaturation step of PCR. This makes it an ideal choice for PCR as it ensures that the enzyme remains active throughout the process.

Why Is Taq Polymerase Used in a Polymerase Chain Reaction