In a scratch wound assay,cells are treated with inhibitors of Rho kinase.What would be observed?
A)The cells completely close the scratch,and only lamellipodia and filapodia are seen with actin staining.
B)The distance the cells migrate into the scratch is small,and actin staining reveals stress fibers,lamellipodia,and filapodia.
C)The cells are stimulated to undergo mitosis.
D)The cells migrate into the scratch,but no stress fibers can be observed with actin staining.

Question

Quizplus · Accepted Answer

Inhibitors of Rho kinase would lead to decreased activity of Rho, a small GTPase that regulates actin cytoskeleton dynamics including formation of stress fibers. Therefore, in the scratch wound assay, cells treated with Rho kinase inhibitors would show reduced migration into the scratch, leading to a smaller distance migrated. Actin staining would reveal the presence of lamellipodia and filapodia, but also stress fibers, reflecting the cell's attempt to migrate despite reduced Rho activity.

In a Scratch Wound Assay,cells Are Treated with Inhibitors of Rho