The rationale for including the plasmid that was not cloned with the linker was
A) the plasmid without the linker served as a positive control to demonstrate that the assay was performing as expected
B) that the plasmid was used to make sure there was no RNA degradation
C) the plasmid was used to make sure there was no DNA degradation
D) the plasmid was included as a carrier to help separate the RNA that was produced from the unincorporated radioactive nucleotides.

Question

Quizplus · Accepted Answer

The plasmid without the linker served as a positive control to demonstrate that the assay was performing as expected. This control ensures that any observed changes in RNA production can be attributed to the presence or absence of the linker and not to experimental error.

The Rationale for Including the Plasmid That Was Not Cloned