The absence of a band when the linker is present at about -25 indicates that
A) This region is critical for the in vitro transcription reaction
B) this region is not critical for the in vitro transcription reaction
C) it is not possible to determine if this region is critical for in vitro transcription
D) the linker interfered with transcription

Question

Quizplus · Accepted Answer

The absence of a band suggests that the presence of the linker at about -25 disrupts the transcription process, indicating that this region is critical for the in vitro transcription reaction.

The Absence of a Band When the Linker Is Present