What is the fluorescence resonance energy transfer (FRET)methodology?
A) The transfer of energy from a donor dye molecule to an acceptor dye molecule.
B) A fluorescent probe is mixed with labeled primers and a sandwich occurs where the fluorescent molecule is activated to fluorescent.
C) A light shines on the fluorescent probe label and it fluoresces.
D) A chromogenic substrate is mixed into solution, and when it combines with the Taq DNA polymerase, a fluorophore is formed and it fluoresces.

Question

Quizplus · Accepted Answer

FRET is the transfer of energy from a donor dye molecule to an acceptor dye molecule; or FRET also occurs between a fluorescent dye and a quenching molecule that keeps emitted light low until the fluorescent dye is released from the quencher.For FRET to occur,the two molecules must be in close proximity to each other-they should be within about 1 to 5 nucleotides of each other.

What Is the Fluorescence Resonance Energy Transfer (FRET)methodology