During the denaturation step in polymerase chain reaction (PCR):
A) DNA polymerase extends the primers.
B) Oligonucleotide primers are hybridized to the single-stranded DNA.
C) Native DNA is cut into small pieces with a restriction enzyme.
D) The target double-stranded DNA is separated into single strands during the denaturation step.

Question

Quizplus · Accepted Answer

Because single-stranded DNA targets are necessary for PCR assays,the target double-stranded DNA is denatured or separated into single strands by raising the temperature to above 90° C,whereas DNA bonds separately.

During the Denaturation Step in Polymerase Chain Reaction (PCR)