What chemical has been very successful in reducing carryover from polymerase chain reaction (PCR) assays?
A)Taq DNA polymerase
B)Uracil-N-glycosylate
C)Thymine
D)Bromthymol blue

Question

Quizplus · Accepted Answer

The PCR amplicon is produced normally, but with uracil substituted for thymine.The enzyme uracil-N-glycosylase prevents replication of uracil-containing DNA.PCR reagents can be preincubated with this enzyme to ensure that amplicon carryover does not contaminate the reagents.

What Chemical Has Been Very Successful in Reducing Carryover from Polymerase