During the denaturation step in polymerase chain reaction (PCR):
A)DNA polymerase extends the primers.
B)oligonucleotide primers are hybridized to the single-stranded DNA.
C)native DNA is cut into small pieces with a restriction enzyme.
D)the target double-stranded DNA is separated into single strands.

Question

Quizplus · Accepted Answer

Because single-stranded DNA targets are necessary for PCR assays, the target double-stranded DNA is denatured or separated into single strands by raising the temperature to above 90° C.

During the Denaturation Step in Polymerase Chain Reaction (PCR)