The Gen-Probe AccuProbe system which technology principle?
A)When two solutions are combined and hybridization occurs between the probe and the target, then the tube is centrifuged to detect the precipitated hybridized target and probes.
B)A single-stranded, chemiluminescent-labeled DNA probe is designed to hybridize to the target organism's ribosomal RNA (rRNA), forming a DNA:RNA duplex.
C)A double-stranded, bioluminescent-labeled DNA probe is designed to hybridize to the target organism's DNA, forming a DNA:DNA duplex.
D)A single-stranded, chemiluminescent-labeled RNA probe is designed to hybridize to the target organism's ribosomal RNA (rRNA), forming an RNA:RNA duplex.

Question

Quizplus · Accepted Answer

A single-stranded, chemiluminescent labeled-DNA probe is designed to hybridize to the target organism's ribosomal RNA (rRNA), forming a DNA:RNA duplex.A detector called a luminometer is used to detect these labeled duplexes.The luminometer gives a reading in relative light units (RLU), and the RLU result for a suspicious culture is compared to a positive cutoff RLU value: any reading over the cutoff value is positive, and readings below are negative.

The Gen-Probe AccuProbe System Which Technology Principle