In a hybridization reaction, the pH affects the:
A)probe's nucleic acids so that they do not lose their hydrogen atoms.
B)breakdown of the oligonucleotide pieces.
C)ability of the mismatches between the probe and the target to duplex.
D)stability of double-stranded nucleic acid molecules in solution.

Question

Quizplus · Accepted Answer

pH affects the stability of double-stranded nucleic acid molecules in solution.An alkaline pH promotes dissociation of double-stranded molecules, whereas acidic pH solutions can depurinate probes and target nucleic acid molecules.

In a Hybridization Reaction, the PH Affects The