The steps for performing the Southern blot test include all the following except:
A) after the labeled probe is hybridized to the specific target, the DNA is electrophoresed again, then dried and read.
B) the DNA is first digested with restriction enzymes.
C) once digested, the DNA is separated and immobilized onto a solid membrane.
D) labeled probe is hybridized to the specific DNA sequence.
Correct Answer:
Verified
Q1: The principle of in-solution hybridization is
A) the
Q2: How do higher probe concentrations affect a
Q5: The target nucleic acid strand is the:
A)one
Q6: Which of the following compounds are used
Q8: A Northern blot is used to detect
A)
Q8: The principle of solid support hybridization (often
Q9: The steps for performing the Northern blot
Q11: In a hybridization reaction, the pH affects
Q13: In situ hybridization, first described in 1969,
Q16: The probe is
A) immobilized on a solid
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