What are the three main steps of PCR, and what is their order in a reaction?
A) Denaturation, extension, polymerisation
B) Annealing, reaction, denaturation
C) Hydrolysis, extension, cleavage
D) Denaturation, annealing, extension
E) Polymerisation, reaction, extension

Question

Quizplus · Accepted Answer

The three main steps of PCR (Polymerase Chain Reaction) in their correct order are: 1) Denaturation, where the double-stranded DNA is heated to separate it into two single strands, 2) Annealing, where primers bind to the specific target sequences on the single-stranded DNA, and 3) Extension, where DNA polymerase synthesizes the new DNA strand by adding nucleotides to the primers.

What Are the Three Main Steps of PCR, and What