You observe a scientist extracting gDNA from an organism. The scientist then states he wants to check this material to determine the expression level of some specific genes in the gDNA he has just extracted and purified. How would you advise this scientist?
A) Because he has extracted gDNA, he should do a Southern blot on his extracted material to detect the genes he is specifically interested in.
B) Because he wants to detect expression levels, he needs to do a northern blot on his extracted material as this is the technique used to semi-quantitatively measure transcript levels.
C) He should do Q-PCR or RT-PCR to produce cDNA that he can then analyse for transcript abundance.
D) If he wants to measure expression levels, he needs to instead extract total mRNA and then do a northern blot.
E) If he wants to measure expression levels, he needs to instead extract total mRNA and then do a Southern blot.

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The Answer of You observe a scientist extracting gDNA from an organism. The scientist then states he wants to check this material to determine the expression level of some specific genes in the gDNA he has just extracted and purified. How would you advise this scientist?
A) Because he has extracted gDNA, he should do a Southern blot on his extracted material to detect the genes he is specifically interested in.
B) Because he wants to detect expression levels, he needs to do a northern blot on his extracted material as this is the technique used to semi-quantitatively measure transcript levels.
C) He should do Q-PCR or RT-PCR to produce cDNA that he can then analyse for transcript abundance.
D) If he wants to measure expression levels, he needs to instead extract total mRNA and then do a northern blot.
E) If he wants to measure expression levels, he needs to instead extract total mRNA and then do a Southern blot.

You Observe a Scientist Extracting GDNA from an Organism