The CRISPR/Cas system is a fast and powerful method to introduce specific mutations into cellular genomes via homologous recombination to study the function of genes and the proteins they encode. Which reagents are necessary for CRISPR/Cas?
A) Antibody to DNA nuclease, Cas9
B) Mutant copy of target gene, guide RNA, and Cas9
C) Antisense RNA complementary to target gene, Cas9
D) siRNA complementary to the target mRNA, Cas9

Question

Quizplus · Accepted Answer

The necessary reagents for CRISPR/Cas are a mutant copy of the target gene, a guide RNA, and Cas9 nuclease. The guide RNA directs the Cas9 nuclease to the specific location in the genome to introduce the desired mutation via homologous recombination. Antibodies and siRNAs are not required for this system. Antisense RNA can be used for gene knockdown but not for introducing mutations via homologous recombination.

The CRISPR/Cas System Is a Fast and Powerful Method to Introduce