What technique would you use to determine if the level of a specific mRNA had been increased in response to an inducer?
A) Northern blotting
B) Southern blotting
C) Western blotting
D) Immunoprecipitation

Question

Quizplus · Accepted Answer

Northern blotting is a technique that allows the detection and quantification of a specific mRNA. It involves the use of a probe that is complementary to the mRNA of interest, which is labeled and then hybridized to the mRNA on a membrane. The level of mRNA can then be determined by measuring the intensity of the signal. This technique is commonly used to study changes in gene expression in response to various stimuli or inducers. Southern blotting is used to detect specific DNA sequences, Western blotting detects proteins, and immunoprecipitation is used to isolate specific proteins.

What Technique Would You Use to Determine If the Level