Which character is not necessary in a plasmid vector to facilitate successful cloning?
A) Origin of replication
B) Gene conferring resistance to antibiotic
C) Restriction site
D) Strong bacterial promoter

Question

Quizplus · Accepted Answer

The origin of replication is necessary for plasmid replication, the gene conferring resistance to antibiotic is useful for the selection of transformed bacteria, and the restriction site(s) allow for the insertion of foreign DNA into the plasmid. However, the presence of a strong bacterial promoter is not necessary for successful cloning as it only serves to drive expression of the inserted DNA in the bacteria.

Which Character Is Not Necessary in a Plasmid Vector to Facilitate