Which sequence correctly outlines the process of RNA interference, beginning with a double-stranded RNA molecule?
A) Unwinding of siRNA; cleavage by Dicer; association with RISC; pairing with target mRNA; mRNA cleavage
B) Reverse transcription of RNA to cDNA; addition of oligonucleotide linkers containing restriction endonuclease cleavage sites; cDNA ligation to appropriate vector; introduction of recombinant molecule into E. Coli
C) Cleavage by Dicer; association with RISC; unwinding of siRNA; pairing with target mRNA; mRNA cleavage
D) Reverse transcription of RNA to cDNA; double-stranded DNA heated to separate strands; cooling to allow primers to bind to each strand of DNA; synthesis of new DNA molecules by Taq polymerase

Question

Quizplus · Accepted Answer

RNA interference begins with the cleavage of double-stranded RNA by the enzyme Dicer, followed by the association of the resulting small interfering RNA (siRNA) with the RNA-induced silencing complex (RISC), unwinding of the siRNA, and finally, the pairing and cleavage of the target mRNA.

Which Sequence Correctly Outlines the Process of RNA Interference, Beginning