Generally speaking, sequence techniques have become so sensitive that if you are able to isolate the protein on a gel, there is enough of it to get a significant amount of its sequence.

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Modern protein sequencing techniques, such as mass spectrometry, have become highly sensitive, allowing for the sequencing of very small amounts of protein that can be isolated on a gel, often requiring only picomoles or less of the protein for successful analysis.

Generally Speaking, Sequence Techniques Have Become So Sensitive That If