Which of the following explains why a secondary antibody is used in an ELISA?
A) two antibodies will be twice as visible as one antibody
B) it allows the visible signal to be amplified
C) primary antibodies cannot be tagged with visible markers
D) none of the choices

Question

Quizplus · Accepted Answer

The use of a secondary antibody in an ELISA (Enzyme-Linked Immunosorbent Assay) allows for the amplification of the signal. This is because multiple secondary antibodies can bind to each primary antibody, each carrying an enzyme or tag that generates a detectable signal, thereby enhancing the sensitivity and visibility of the assay.

Which of the Following Explains Why a Secondary Antibody Is