Which of the following is a similarity between ion-exchange chromatography and affinity chromatography?
A) Both use a column resin that binds the protein of interest.
B) Both involve a less-specific interaction and are based on net charge.
C) Both separate molecules of varying molecular weights.
D) Both use very high-resolution columns that can run under high pressures.

Question

Quizplus · Accepted Answer

Both ion-exchange chromatography and affinity chromatography utilize a column resin specifically designed to bind the protein of interest, albeit through different mechanisms (charge interactions in ion-exchange and specific binding interactions in affinity chromatography).

Which of the Following Is a Similarity Between Ion-Exchange Chromatography