Indicate whether each of the following descriptions better applies to Illumina sequencing (I), Ion Torrent™ sequencing (T), or both sequencing technologies (B). Your answer would be a four-letter string composed of letters B, I, and T only, e.g. IBBB.
( ) It uses fluorescently labeled nucleotides.
( ) It uses PCR-generated copies of DNA.
( ) It is a second-generation sequencing technology and employs a cyclic wash-and-measure paradigm.
( ) It relies on the fidelity of DNA polymerase for its accuracy.

Question

Indicate whether each of the following descriptions better applies to Illumina sequencing (I), Ion Torrent™ sequencing (T), or both sequencing technologies (B). Your answer would be a four-letter string composed of letters B, I, and T only, e.g. IBBB.
( )	It uses fluorescently labeled nucleotides.
( )	It uses PCR-generated copies of DNA.
( )	It is a second-generation sequencing technology and employs a cyclic wash-and-measure paradigm.
( )	It relies on the fidelity of DNA polymerase for its accuracy.

Quizplus · Accepted Answer

Both of them are second-generation sequencing technologies and require DNA amplification before sequencing-by-synthesis, which involves cycles of wash and measurement. Illumina sequencing uses fluorescent tags, but Ion Torrent sequencing measures pH changes.

Indicate Whether Each of the Following Descriptions Better Applies to Illumina