You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the gene is provided below. Which following pair of primers would direct the amplification of this gene? All sequences are written from 5? to 3?.

ATGAAATCTACGTTTCAC……CCCCCAGTACCCCCCTTA

A) ATGAAATCTACGTTTCAC ; CCCCCAGTACCCCCCTTA
B) ATGAAATCTACGTTTCAC ; TAAGGGGGGTACTGGGGG
C) TACTTTAGATGCAAAGTG; ATTCCCCCCATGACCCCC
D) TACTTTAGATGCAAAGTG ; CCCCCAGTACCCCCCTTA
E) GTGAAACGTAGATTTCAT; ATTCCCCCCATGACCCCC

Question

You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the gene is provided below. Which following pair of primers would direct the amplification of this gene? All sequences are written from 5? to 3?.

ATGAAATCTACGTTTCAC……CCCCCAGTACCCCCCTTA

ATGAAATCTACGTTTCAC……CCCCCAGTACCCCCCTTA

A) ATGAAATCTACGTTTCAC ; CCCCCAGTACCCCCCTTA
B) ATGAAATCTACGTTTCAC ; TAAGGGGGGTACTGGGGG
C) TACTTTAGATGCAAAGTG; ATTCCCCCCATGACCCCC
D) TACTTTAGATGCAAAGTG ; CCCCCAGTACCCCCCTTA
E) GTGAAACGTAGATTTCAT; ATTCCCCCCATGACCCCC

Quizplus · Accepted Answer

The first (forward) primer would hybridize to the strand that is complementary to that shown (i.e. coding strand) and would be extended toward the right, while the second (reverse) primer would hybridize to the coding strand and would be extended toward the left.

You Would Like to Clone a Gene by PCR