A researcher has labeled the 5? end of a DNA primer with ³²P radioisotopes. The primer can hybridize to a complementary sequence near the 3? end of a long, spliced mRNA molecule composed of three exons. The mRNA molecule can interact with two RNA-binding proteins: one of them (protein 1) binds to the first exon and the other (protein 2) binds to the second exon. The researcher uses reverse transcriptase to extend the primer in the presence of the mRNA and either of these proteins, then separates the cDNA products by gel electrophoresis, and finally visualizes the labeled DNA bands by autoradiography. The results are presented schematically in the following drawing. Assuming that the reverse transcriptase falls off the template when confronted by bound proteins, which lane (A or B) in the gel corresponds to the reaction in the presence of protein 1? Write down A or B as your answer.

Question

Quizplus · Accepted Answer

Binding of protein 1 to a region of mRNA that is further upstream allows the reverse transcriptase enzyme to further extend the labeled primer (create a longer DNA) before reaching a bound protein.

A Researcher Has Labeled the 5? End of a DNA