The AgeI restriction enzyme recognizes the sequence ACCGGT and cuts each strand between the first (A) and the second (C) nucleotide. This can be represented as A|CCGGT, with the vertical bar indicating the cleavage position. If a DNA fragment is cut with AgeI at both ends, cutting a plasmid with which of the following enzymes would then allow insertion of the fragment into the plasmid by simple ligation of sticky ends?
A) FseI, with the recognition sequence GGCCGG|CC
B) AatI, with the recognition sequence AGG|CCT
C) BstZI, with the recognition sequence C|GGCCG
D) MroI, with the recognition sequence T|CCGGA
E) None of the above

Question

Quizplus · Accepted Answer

Both AgeI and MroI generate the same sticky end (with the sequence CCGG) as a 5′ overhang after cleavage.

The AgeI Restriction Enzyme Recognizes the Sequence ACCGGT and Cuts